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SARS-CoV-2 by PCR

Order Name SARS-CoV2
Test Number: 6907557
Revision Date 07/16/2020
Test Name Methodology LOINC Code
SARS-CoV-2 by PCR
Nucleic Acid Detection 94500-6 
SARS-CoV-2 Source
Prompt 31208-2 
SPECIMEN REQUIREMENTS
Specimen Specimen Volume (min) Specimen Type Specimen Container Transport Environment
Preferred 1-3mL (0.8mL) See Instructions See Instructions See Instructions
Instructions
  1. Specimen sites: Nasopharyngeal (NP), Oropharyngeal (OP), Deep Nasal/Mid-turbinate (MT) or Anterior Nares (AN) swab.
    If collecting MT or AN specimens , both nares should be generously sampled with a single swab and placed in a single tube to maximize virus particle collection. NP washes/aspirates and bronchial washings or bronchoalveolar lavage (BAL) specimens collected with a minimal volume of sterile saline (2 mL or less) are also acceptable.
  2. Swab types: Any swab of appropriate size and configuration with a synthetic or rayon tip and plastic shaft can be used.
    Do NOT submit swabs with calcium alginate tips or swabs with wood shafts.
       (a) APTIMA Multitest Swab kit: OP, MT or AN site collections (swab too large for NP site)
        - Transport at room temperature or refrigerated; Do not freeze; Stability: 2- 30'C up to 6 days.
      (b) All other swab types: Suitable for all specimen sites listed, depending on size of swab
        - Refrigerate specimens if they will be received in lab within 72 hours of collection.
           If there will be delayed transport, freeze specimen at -20'C.
  3. Media types: VTM, UTM, M6, ESwab, or sterile saline or Aptima Multitest Swab kit media
  4. Swabs must be placed in 1-3 mL of liquid media, broken or cut at the top of the tube, and tubes tightly capped. Specimen tubes should be labeled with two unique patient identifiers. Place the tube containing the swab in a biohazard bag and submit with paper requisition or place electronic order. Please keep specimen refrigerated until pick-up.
  5. Causes for rejection: Swabs with calcium alginate tips; swabs with wooden shafts; refrigerated samples greater than 72 hours old; room temperature swabs greater than 24 hours old; improperly labeled; grossly contaminated; broken or leaking tube; collection with substances inhibitory to PCR including heparin, hemoglobin, ethanol, EDTA concentrations >0.01M; unacceptable transport media.
  6. Couriers will transport to Regional Medical Core Laboratory. Call RML at 918-744-2232 to request specimen pickup if not routinely scheduled at your location.
GENERAL INFORMATION
Testing Schedule Daily 
Expected TAT 48-96 hours from receipt of specimen in lab.  
Clinical Use Detects the 2019 novel coronavirus (SARS-CoV-2). 
Notes Please be aware SARS CoV2 PCR volumes are increasing daily and due to the significant supply constraints caused by the resurgence of Covid throughout the United States, our vendors have been challenged and unable to ship us our orders of reagents in totality and are providing reduced allocations at this time. This is a nation-wide issue that all labs are experiencing. We do have additional resources being sent to us, but our current TAT is 48-96 hours with critical patients receiving highest priority for testing. Specimens for patients needing elective surgery should have specimen collected 72 hours in advance of the scheduled surgery. Please check the Coronavirus testing page often for updates as RML is continually updating the testing process as this situation develops.
Click Here to view the Coronavirus testing page.
CPT Code(s) CPT: 87635
HCPCS: U0004
Lab Section Molecular Diagnostic